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dnase digestion step  (Qiagen)


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    Qiagen dnase digestion step
    Dnase Digestion Step, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 26841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnase+digestion+step/RNase-Free+DNase+Set/bio_rxiv__64898__2026__04__07__717049-181-18-21
    Average 99 stars, based on 26841 article reviews
    dnase digestion step - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: SLAMF1-peptide mediated epigenetic priming reprograms innate immune responses in sepsis
    Article Snippet: Secondary antibodies (HRP-linked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S (Glostrup, Denmark). .. Total RNA was isolated from the cells using Qiazol reagent (#79306, QIAGEN, Germantown, AR, USA), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254, QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a quantitative real-time polymerase-chain reaction (RT-qPCR) (#K1641, ThermoFisher Scientific, Waltham, MA, USA), in accordance with the protocol of the manufacturer, from 400–600 ng of total RNA per sample. .. Q-PCR was performed using the PerfeCTa qPCR FastMix (#733-1393, Quanta Biosciences, Gaithersburg, MD, USA) in replicates and cycled in a StepOnePlusTM Real-Time PCR cycler (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: Glucokinase activity suppresses hepatic cholesterol synthesis and triglyceride accumulation: A new model for the effects of the GKRP P466L common human variant
    Article Snippet: .. Total RNA was isolated from frozen liver samples using the RNeasy Plus kit (Qiagen Cat# 74134), including the DNase digestion step (Qiagen Cat #79254). .. Complementary DNA was generated using M-MuLV reverse transcriptase and relative gene expression was quantified by real time qPCR using the SYBR Green dye-based assay (ABI).

    Article Title: A novel TIRAP-MyD88 inhibitor blocks TLR7- and TLR8-induced type I IFN responses.
    Article Snippet: Secondary Abs (HRPlinked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S, and donkey antigoat (#A16005) and donkey anti-sheep (#A1604) were from Invitrogen (Thermo Fisher Scientific). .. Real-time quantitative polymerase chain reaction Total RNA was isolated from the cells using QIAzol reagent (#79306; QIAGEN), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254; QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a real-time quantitative polymerase chain reaction (qPCR) (#K1641; Thermo Fisher Scientific), in accordance with the protocol of the manufacturer, from 400 to 600 ng of total RNA per sample. qPCR was performed using the PerfeCTa qPCR FastMix (#733-1393; Quanta Biosciences) in replicates and cycled in a StepOnePlus Real-Time PCR cycler (Thermo Fisher Scientific). .. The following TaqMan Gene Expression Assays (Applied Biosystems, Thermo Fisher Scientific) were used: IFNβ (Hs01077958_s1), TNF (Hs00174128_m1), TBP (Hs00427620_m1), IL-6 (Hs00985639_m1), and IL-1β (Hs01555410_m1) for human cells; Ifnβ (Mm00439552_s1), Tnf (Mm00443258_m1), Il-1β (Mm00434228_m1), and Tbp (Mm01277042_m1) for murine cells.

    Extraction:

    Article Title: SLAMF1-peptide mediated epigenetic priming reprograms innate immune responses in sepsis
    Article Snippet: Secondary antibodies (HRP-linked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S (Glostrup, Denmark). .. Total RNA was isolated from the cells using Qiazol reagent (#79306, QIAGEN, Germantown, AR, USA), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254, QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a quantitative real-time polymerase-chain reaction (RT-qPCR) (#K1641, ThermoFisher Scientific, Waltham, MA, USA), in accordance with the protocol of the manufacturer, from 400–600 ng of total RNA per sample. .. Q-PCR was performed using the PerfeCTa qPCR FastMix (#733-1393, Quanta Biosciences, Gaithersburg, MD, USA) in replicates and cycled in a StepOnePlusTM Real-Time PCR cycler (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: A novel TIRAP-MyD88 inhibitor blocks TLR7- and TLR8-induced type I IFN responses.
    Article Snippet: Secondary Abs (HRPlinked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S, and donkey antigoat (#A16005) and donkey anti-sheep (#A1604) were from Invitrogen (Thermo Fisher Scientific). .. Real-time quantitative polymerase chain reaction Total RNA was isolated from the cells using QIAzol reagent (#79306; QIAGEN), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254; QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a real-time quantitative polymerase chain reaction (qPCR) (#K1641; Thermo Fisher Scientific), in accordance with the protocol of the manufacturer, from 400 to 600 ng of total RNA per sample. qPCR was performed using the PerfeCTa qPCR FastMix (#733-1393; Quanta Biosciences) in replicates and cycled in a StepOnePlus Real-Time PCR cycler (Thermo Fisher Scientific). .. The following TaqMan Gene Expression Assays (Applied Biosystems, Thermo Fisher Scientific) were used: IFNβ (Hs01077958_s1), TNF (Hs00174128_m1), TBP (Hs00427620_m1), IL-6 (Hs00985639_m1), and IL-1β (Hs01555410_m1) for human cells; Ifnβ (Mm00439552_s1), Tnf (Mm00443258_m1), Il-1β (Mm00434228_m1), and Tbp (Mm01277042_m1) for murine cells.

    Purification:

    Article Title: SLAMF1-peptide mediated epigenetic priming reprograms innate immune responses in sepsis
    Article Snippet: Secondary antibodies (HRP-linked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S (Glostrup, Denmark). .. Total RNA was isolated from the cells using Qiazol reagent (#79306, QIAGEN, Germantown, AR, USA), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254, QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a quantitative real-time polymerase-chain reaction (RT-qPCR) (#K1641, ThermoFisher Scientific, Waltham, MA, USA), in accordance with the protocol of the manufacturer, from 400–600 ng of total RNA per sample. .. Q-PCR was performed using the PerfeCTa qPCR FastMix (#733-1393, Quanta Biosciences, Gaithersburg, MD, USA) in replicates and cycled in a StepOnePlusTM Real-Time PCR cycler (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: A novel TIRAP-MyD88 inhibitor blocks TLR7- and TLR8-induced type I IFN responses.
    Article Snippet: Secondary Abs (HRPlinked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S, and donkey antigoat (#A16005) and donkey anti-sheep (#A1604) were from Invitrogen (Thermo Fisher Scientific). .. Real-time quantitative polymerase chain reaction Total RNA was isolated from the cells using QIAzol reagent (#79306; QIAGEN), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254; QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a real-time quantitative polymerase chain reaction (qPCR) (#K1641; Thermo Fisher Scientific), in accordance with the protocol of the manufacturer, from 400 to 600 ng of total RNA per sample. qPCR was performed using the PerfeCTa qPCR FastMix (#733-1393; Quanta Biosciences) in replicates and cycled in a StepOnePlus Real-Time PCR cycler (Thermo Fisher Scientific). .. The following TaqMan Gene Expression Assays (Applied Biosystems, Thermo Fisher Scientific) were used: IFNβ (Hs01077958_s1), TNF (Hs00174128_m1), TBP (Hs00427620_m1), IL-6 (Hs00985639_m1), and IL-1β (Hs01555410_m1) for human cells; Ifnβ (Mm00439552_s1), Tnf (Mm00443258_m1), Il-1β (Mm00434228_m1), and Tbp (Mm01277042_m1) for murine cells.

    cDNA Synthesis:

    Article Title: SLAMF1-peptide mediated epigenetic priming reprograms innate immune responses in sepsis
    Article Snippet: Secondary antibodies (HRP-linked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S (Glostrup, Denmark). .. Total RNA was isolated from the cells using Qiazol reagent (#79306, QIAGEN, Germantown, AR, USA), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254, QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a quantitative real-time polymerase-chain reaction (RT-qPCR) (#K1641, ThermoFisher Scientific, Waltham, MA, USA), in accordance with the protocol of the manufacturer, from 400–600 ng of total RNA per sample. .. Q-PCR was performed using the PerfeCTa qPCR FastMix (#733-1393, Quanta Biosciences, Gaithersburg, MD, USA) in replicates and cycled in a StepOnePlusTM Real-Time PCR cycler (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: A novel TIRAP-MyD88 inhibitor blocks TLR7- and TLR8-induced type I IFN responses.
    Article Snippet: Secondary Abs (HRPlinked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S, and donkey antigoat (#A16005) and donkey anti-sheep (#A1604) were from Invitrogen (Thermo Fisher Scientific). .. Real-time quantitative polymerase chain reaction Total RNA was isolated from the cells using QIAzol reagent (#79306; QIAGEN), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254; QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a real-time quantitative polymerase chain reaction (qPCR) (#K1641; Thermo Fisher Scientific), in accordance with the protocol of the manufacturer, from 400 to 600 ng of total RNA per sample. qPCR was performed using the PerfeCTa qPCR FastMix (#733-1393; Quanta Biosciences) in replicates and cycled in a StepOnePlus Real-Time PCR cycler (Thermo Fisher Scientific). .. The following TaqMan Gene Expression Assays (Applied Biosystems, Thermo Fisher Scientific) were used: IFNβ (Hs01077958_s1), TNF (Hs00174128_m1), TBP (Hs00427620_m1), IL-6 (Hs00985639_m1), and IL-1β (Hs01555410_m1) for human cells; Ifnβ (Mm00439552_s1), Tnf (Mm00443258_m1), Il-1β (Mm00434228_m1), and Tbp (Mm01277042_m1) for murine cells.

    Real-time Polymerase Chain Reaction:

    Article Title: SLAMF1-peptide mediated epigenetic priming reprograms innate immune responses in sepsis
    Article Snippet: Secondary antibodies (HRP-linked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S (Glostrup, Denmark). .. Total RNA was isolated from the cells using Qiazol reagent (#79306, QIAGEN, Germantown, AR, USA), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254, QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a quantitative real-time polymerase-chain reaction (RT-qPCR) (#K1641, ThermoFisher Scientific, Waltham, MA, USA), in accordance with the protocol of the manufacturer, from 400–600 ng of total RNA per sample. .. Q-PCR was performed using the PerfeCTa qPCR FastMix (#733-1393, Quanta Biosciences, Gaithersburg, MD, USA) in replicates and cycled in a StepOnePlusTM Real-Time PCR cycler (ThermoFisher Scientific, Waltham, MA, USA).

    Article Title: A novel TIRAP-MyD88 inhibitor blocks TLR7- and TLR8-induced type I IFN responses.
    Article Snippet: Secondary Abs (HRPlinked) swine anti-rabbit (#P0399) and goat anti-mouse (#P0447) were from DAKO Denmark A/S, and donkey antigoat (#A16005) and donkey anti-sheep (#A1604) were from Invitrogen (Thermo Fisher Scientific). .. Real-time quantitative polymerase chain reaction Total RNA was isolated from the cells using QIAzol reagent (#79306; QIAGEN), and chloroform extraction was followed by purification on RNeasy Mini columns (#74106) with DNAse digestion step (#79254; QIAGEN). cDNA was prepared with a Maxima First Strand cDNA Synthesis Kit for a real-time quantitative polymerase chain reaction (qPCR) (#K1641; Thermo Fisher Scientific), in accordance with the protocol of the manufacturer, from 400 to 600 ng of total RNA per sample. qPCR was performed using the PerfeCTa qPCR FastMix (#733-1393; Quanta Biosciences) in replicates and cycled in a StepOnePlus Real-Time PCR cycler (Thermo Fisher Scientific). .. The following TaqMan Gene Expression Assays (Applied Biosystems, Thermo Fisher Scientific) were used: IFNβ (Hs01077958_s1), TNF (Hs00174128_m1), TBP (Hs00427620_m1), IL-6 (Hs00985639_m1), and IL-1β (Hs01555410_m1) for human cells; Ifnβ (Mm00439552_s1), Tnf (Mm00443258_m1), Il-1β (Mm00434228_m1), and Tbp (Mm01277042_m1) for murine cells.



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